Composite

Part:BBa_K2558214:Design

Designed by: Tsinghua 2018   Group: iGEM18_Tsinghua   (2018-10-05)

Neon lux positive feedback device with luxpR-HS promotor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2026
    Illegal NheI site found at 2049
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 801
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 950
    Illegal SapI.rc site found at 1129


Design Note

Assisted with the experiences we gained form the experiments above, we built and tuned the NEON system. We designed this experiment to characterize how Neon the positive feedback plasmid (BBa_K2558214), and Safety Catch the CRISPRi plasmid (BBa_K2558215, BBa_K2558216) work together.

Source

References